The traditional wright's stain, an alcoholic solution of methylene blue and eosin Y, dates from the early 1890's. Since then there have been many modifications, most involving partial oxidative demethylation of the methylene blue to improve polychroming. Modern day samples of the dye usually contain mixtures of methylene blue, azure A, and thionin ( the mixture is often called "polychromed methylene blue") compounded with eosin Y. Med Chem's Wright's Dip Stat has the azures and the eosin Y in separate solutions which improves staining control and reproducibility as well as speed. The total staining time is about thirty seconds.
|301||Tinted Methanol||8 oz, 16 oz, 1 gal|
|302||Eosinate Stain||8 oz, 16 oz, 1 gal|
|303||Polychromed Methylene Blue||8 oz, 16 oz, 1 gal|
|9265B||Deionized Water||1 gal, 2.5 gal, 5 gal|
Use capillary blood or freshly drawn venous blood that has not been treated with anticoagulants. Hemolysis will render the sample unsatisfactory.
* Staining times vary with the thickness of the slide. Exact staining time should be established by each laboratory.