Ova and Parasite (O&P) Examinations
Introduction |
Macroscopic |
Microscopic |
Microscopic Examination - Ova and Parasite Examination
Introduction |
Direct Wet Smear |
Concentration |
Permanent Stained Smear
Concentration
Introduction |
Formulas |
Quality Control |
Procedures |
Results and Reporting |
Procedure Notes |
Procedure Limitations |
Procedure Review |
Tips and Pitfalls
Tips and Pitfalls
- Centrifugation speed (formalin/ethyl acetate concentration) is critical for the adequate recovery of organisms. Recommended time/speed is 10 min. at 500 Xg; this will allow recovery of all protozoa, including coccidian oocysts and microsporidian spores.
- Remember: operculated eggs (trematode, Diphyllobothrium latum) and heavy eggs(unfertilized Ascaris lumbricoides eggs) will not float using the zinc sulfate flotation concentration method.
- If using ethyl acetate and plastic tubes, remember to clean out the excess ethyl acetate from the inside of the tube with a cotton-tipped swab before making a wet mount. This will prevent excess ethyl acetate from giving a "bubbly" appearance under the coverslip. Wiping the inside of the tube is not as critical when using glass tubes.
