Ova and Parasite (O&P) Examinations
Introduction |
Macroscopic |
Microscopic |
Microscopic Examination - Ova and Parasite Examination
Introduction |
Direct Wet Smear |
Concentration |
Permanent Stained Smear
Permanent Stained Smear
Introduction |
Trichrome Stain |
Iron Hematoxylin Stain |
Modified Iron Hematoxylin Stain |
Procedure Review
Modified Iron Hematoxylin Stain
Introduction |
Formulas |
Procedures |
Procedure Notes |
Procedures
- Prepare slide.
- Place 1 drop of Mayer's albumin on a labeled slide.
- Mix the sediment from the SAF concentration well with an applicator stick.
- Add approximately 1 drop of the fecal concentrate to the albumin and spread the mixture over the slide.
- Allow slide to air dry at room temperature (smear will appear opaque when dry).
- Place slide in 70% alcohol for 5 min.
- Wash in container (not running water) of tap water for 2 min.
- Place slide in carbol fuchsin stain for 5 min.
- Wash slide in running tap water (constant stream of water into container) for 1 min.
- Place slide in acid-alcohol decolorizer for 4 min.*
- Wash slide in running tap water (constant stream of water into container) for 1 min.
- Place slide in iron hematoxylin working solution for 8 min.
- Wash slide in distilled water (in container) for 1 min.
- Place slide in picric acid solution for 3 to 5 min.
- Wash slide in running tap water (constant stream of water into container) for 10 min.
- Place slide in 70% alcohol plus ammonia for 3 min.
- Place slide in 95% alcohol for 5 min.
- Place slide in 100% alcohol for 5 min.
- Place slide in two changes of xylene for 5 min.
*This step can also be performed as follows.
- Place slide in acid-alcohol decolorizer for 2 min.
- Wash slide in running tap water (constant stream of water into container) for 1 min.
- Place slide in acid-alcohol decolorizer for 2 min.
- Wash slide in running tap water (constant stream of water into container) for 1 min.
- Continue staining sequence with step 9 (iron hematoxylin working solution).