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Ova and Parasite (O&P) Examinations

Introduction | Macroscopic | Microscopic |

Microscopic Examination - Ova and Parasite Examination

Introduction | Direct Wet Smear | Concentration | Permanent Stained Smear

Permanent Stained Smear

Introduction | Trichrome Stain | Iron Hematoxylin Stain | Modified Iron Hematoxylin Stain | Procedure Review

Modified Iron Hematoxylin Stain

Introduction | Formulas | Procedures | Procedure Notes |

Procedures

  1. Prepare slide.
    1. Place 1 drop of Mayer's albumin on a labeled slide.
    2. Mix the sediment from the SAF concentration well with an applicator stick.
    3. Add approximately 1 drop of the fecal concentrate to the albumin and spread the mixture over the slide.
  2. Allow slide to air dry at room temperature (smear will appear opaque when dry).
  3. Place slide in 70% alcohol for 5 min.
  4. Wash in container (not running water) of tap water for 2 min.
  5. Place slide in carbol fuchsin stain for 5 min.
  6. Wash slide in running tap water (constant stream of water into container) for 1 min.
  7. Place slide in acid-alcohol decolorizer for 4 min.*
  8. Wash slide in running tap water (constant stream of water into container) for 1 min.
  9. Place slide in iron hematoxylin working solution for 8 min.
  10. Wash slide in distilled water (in container) for 1 min.
  11. Place slide in picric acid solution for 3 to 5 min.
  12. Wash slide in running tap water (constant stream of water into container) for 10 min.
  13. Place slide in 70% alcohol plus ammonia for 3 min.
  14. Place slide in 95% alcohol for 5 min.
  15. Place slide in 100% alcohol for 5 min.
  16. Place slide in two changes of xylene for 5 min.

*This step can also be performed as follows.

    1. Place slide in acid-alcohol decolorizer for 2 min.
    2. Wash slide in running tap water (constant stream of water into container) for 1 min.
    3. Place slide in acid-alcohol decolorizer for 2 min.
    4. Wash slide in running tap water (constant stream of water into container) for 1 min.
    5. Continue staining sequence with step 9 (iron hematoxylin working solution).