Ova and Parasite (O&P) Examinations
Introduction |
Macroscopic |
Microscopic |
Microscopic Examination - Ova and Parasite Examination
Introduction |
Direct Wet Smear |
Concentration |
Permanent Stained Smear
Permanent Stained Smear
Introduction |
Trichrome Stain |
Iron Hematoxylin Stain |
Modified Iron Hematoxylin Stain |
Procedure Review
Modified Iron Hematoxylin Stain
Introduction |
Formulas |
Procedures |
Procedure Notes |
Formulas
Mayer's albumin
Add an equal quantity of glycerin to a fresh egg white. Mix gently and thoroughly. Store at 4°C and indicate an expiration date of 3 months. Mayer's albumin from commercial suppliers can normally be stored at 25°C for 1 year [e.g., product 756, E. M. Diagnostic Systems Inc., 480 Democrat Road, Gibbstown, NJ 08027; (800) 443?3637].
Stock solution of hematoxylin stain
- Hematoxylin powder 10 g
- Ethanol (95% or 100%) 1,000 ml
- Mix well until dissolved.
- Store in a clear glass bottle in a light area. Allow to ripen for 14 days before use.
- Store at room temperature with an expiration date of 1 year.
Mordant
- Ferrous ammonium sulfate [Fe(NH4)2(SO4)2 6H2O] 10 g
- Ferric ammonium sulfate [FeNH4(SO4)2 12H2O] 10 g
- Hydrochloric acid (concentrated) 10 ml
- Distilled water to 1,000 ml
Working solution of hematoxylin stain
- Mix equal quantities of stock solution of stain and mordant.
- Allow mixture to cool thoroughly before use (prepare at least 2 h prior to use). The working solution should be made fresh every week.
Picric acid
Mix equal quantities of distilled water and an aqueous saturated solution of picric acid to make a 50% saturated solution.
Acid-alcohol decolorizer
- Hydrochloric acid (concentrated) 30 ml
- Alcohol to 1,000 ml
70% alcohol and ammonia
- 70% alcohol 50 ml
- Ammonia 0.5-1.0 ml
Add enough ammonia to bring the pH to approximately 8.0.
Carbol fuchsin
- To make basic fuchsin (solution A), dissolve 0.3 g of basic fuchsin in 10 ml of 95% ethanol.
- To make phenol (solution B), dissolve 5 g of phenol crystals in 100 ml of distilled water. (Gentle heat may be needed.)
- Mix solution A with solution B.
- Store at room temperature. Solution is stable for 1 year.
