Ova and Parasite (O&P) Examinations
Introduction |
Macroscopic |
Microscopic |
Microscopic Examination - Ova and Parasite Examination
Introduction |
Direct Wet Smear |
Concentration |
Permanent Stained Smear
Permanent Stained Smear
Introduction |
Trichrome Stain |
Iron Hematoxylin Stain |
Modified Iron Hematoxylin Stain |
Procedure Review
Trichrome Stain
Introduction |
Formulas |
Quality Control |
Procedures |
Results and Reporting |
Procedure Notes |
Procedure Limitations |
Formulas
Trichrome stain
- Chromotrope 2R 0.6 g
- Light green SF 0.3 g
- Phosphotungstic acid 0.7 g
- Acetic acid (glacial) 1.0 ml
- Distilled water 100 ml
- Prepare the stain by adding 1.0 ml of acetic acid to the dry components. Allow the mixture to stand (ripen) for 15 to 30 min at room temperature.
- Add 100 ml of distilled water. Properly prepared stain will be purple in color.
- Store in a glass or plastic bottle at room temperature. The shelf life is 24 months.
70% ethanol plus iodine
- Prepare a stock solution by adding iodine crystals to 70% alcohol until a dark solution is obtained (1 to 2 g/100 ml).
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- To use, dilute the stock solution with 70% alcohol until a dark reddish brown or strong tea color is obtained. As long as the color is acceptable, new working solution does not have to be replaced. Replacement time will depend on the number of smears stained and the size of the container (1 week to several weeks).
90% ethanol, acidified
- 90% ethanol 99.5 ml
- Acetic acid (glacial) 0.5 ml
- Prepare by combining
- 70% isopropyl or ethyl alcohol 100% ethyl alcohol (recommended) or 95% ethyl alcohol (second choice)
- Xylene or xylene substitute
