Ova and Parasite (O&P) Examinations
Introduction |
Macroscopic |
Microscopic |
Microscopic Examination - Ova and Parasite Examination
Introduction |
Direct Wet Smear |
Concentration |
Permanent Stained Smear
Permanent Stained Smear
Introduction |
Trichrome Stain |
Iron Hematoxylin Stain |
Modified Iron Hematoxylin Stain |
Procedure Review
Iron Hematoxylin Stain
Introduction |
Formulas |
Procedures |
Results and Reporting |
Procedure Notes |
Procedure Limitations |
Formulas
Solution 1
- Hematoxylin (crystal or powder) 10 g
- Ethanol (absolute) 1,000 ml
Place solution in a stoppered clear flask or bottle and allow to ripen in a lighted room for at least 1 week at room temperature.
Solution 2
- Ferrous ammonium sulfate [Fe(NH4)2(SO4)2 6H2O] 10 g
- Ferric ammonium sulfate [FeNH4(SO4)2 12H2O] 10 g
- Hydrochloric acid (concentrated) 10 ml
- Distilled water 1,000 ml
Working solution
Mix equal volumes of solutions 1 and 2. The working solution should be made fresh every week.
70% ethanol plus iodine
- Prepare a stock solution by adding iodine crystals to 70% alcohol until a dark solution is obtained (1 to 2 g/100 ml).
- To use, dilute the stock solution with 70% alcohol until a dark reddish brown (port wine) or strong tea color is obtained. As long as the color is acceptable, new working solution does not have to be replaced. Replacement time will depend on the number of smears stained and the size of the container (1 week to several weeks).
90% ethanol, acidified
- 90% ethanol 99.5 ml
- Acetic acid (glacial) 0.5 ml
Prepare by combining.
70% isopropyl or ethyl alcohol 100% ethyl alcohol (recommended) or 95% ethyl alcohol (second choice)
Xylene or xylene substitute
