Ova and Parasite (O&P) Examinations
Introduction |
Macroscopic |
Microscopic |
Microscopic Examination - Ova and Parasite Examination
Introduction |
Direct Wet Smear |
Concentration |
Permanent Stained Smear
Direct Wet Smear
Introduction |
Formulas |
Quality Control |
Procedures |
Results and Reporting |
Procedure Notes |
Procedure Limitations |
Procedure Review
Formulas
Saline (0.85% NaCl)
- NaCl 0.85 g
- Distilled water 100.0 ml
- Dissolve the sodium chloride in distilled water in a flask or bottle, using a magnetic stirrer.
- Distribute 10 ml into each of 10 screw?cap tubes.
- Label as 0.85% NaCl with an expiration date of 1 year.
- Sterilize by autoclaving at 121 C for 15 min.
- When cool, store at 4 C.
D'Antoni's Iodine
- Potassium iodide 1.0 g
- Powdered iodine crystals 1.5 g
- Distilled water 100.0 ml
- Dissolve the potassium iodide and iodine crystals in distilled water in a flask or bottle, using a magnetic stirrer.
- The potassium iodide solution should be saturated with iodine, with some excess crystals left on the bottom of the bottle.
- Store in a brown, glass-stoppered bottle at room temperature and in the dark.
- This stock solution is ready for immediate use. Label as D'Antoni's iodine with an expiration date of 1 year (the stock solution remains good as long as an excess of iodine crystals remain on the bottom of the bottle).
- Aliquot some of the iodine into a brown dropper bottle. The working solution should resemble a strong tea color and should be discarded when it lightens in color (usually within 10 to 14 days). Note: The stock and working solution formulas are identical, but the stock solution is held in the dark and will retain the strong tea color, while the working solution will fade and have to be periodically replaced.
