- 1.1 Parasite Classification |
- 1.2 Body Site, Specimens, Procedures, Parasites, Comments |
- 1.3 STAT Testing in Parasitology |
- 1.4 Test Issues and Reports: Computer Report Comments|
- 1.5 Rapid Diagnostic Testing
- 2.1 Stool Testing Order Recommendations |
- 2.2 Fecal specimens for parasites: options for collection and processinga2 |
- 2.3 Preservatives used for Stool Specimens
- 3.1 Body Sites and Specimen Collection | - 3.2 Body sites and the most common parasites recovered | - 3.3 Body Site, Specimens and Recommended Stain | - 3.4 Examination of tissues and body fluids | - 3.5 Parasitic Infections: Clinical Findings Healthy/Compromised Hosts | - 3.6 Microscope Calibration | - 3.7 Serologic, Antigen, and Probe Tests for Parasite Diagnosis
- 4.1 Protozoa: Intestinal Tract, Urogenital System: Key Characteristics | - 4.2 Tissue Protozoa: Characteristics |
- 4.3 Tips on Performance of Fecal Immunoassays for Intestinal Protozoa
5.1 Helminths: Key Characteristics | 5.2 Helminth Parasites Associated with Eosinophilia
6.1 Reference Laboratory for Parasite Blood Testing | 6.2 Parasites Found in Blood: Characteristics
7.1 Malaria (5 Species) (2 P. ovale subspecies) | 7.2 Malaria (5 Species, Images) | 7.3 Rapid Malaria Testing (BinaxNOW Malaria Test) | 7.4 Malaria Parasitemia Method |
7.5 Malaria Parasitemia Interpretation
- USE OF A REFERENCE LABORATORY FOR PARASITE BLOOD DIAGNOSTIC
TESTING (Including the Binax Rapid Test and Report Comments)
- HELMINTH PARASITES ASSOCIATED WITH EOSINOPHILIA |
- Histology: Staining Characteristics - Table 1 |
- Histological Identification of Parasites - Table 2 |
- Microscope Calibration |
- Figures for Histology Identification Table 2 |
3.5 Parasitic infections: clinical findings in healthy and compromised hosts |
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Organism(s) |
Clinical findings in: |
|
Healthy host |
Compromised hosta |
|
Entamoeba histolytica |
Asymptomatic to chronic or acute colitis; extraintestinal disease may occur (primary site: right upper lobe of liver) |
Diminished immune capacity may lead to extraintestinal disease. |
Free-living amebae |
Patients tend to have eye infections with Acanthamoeba spp.; linked to poor eye care (primarily contact lens wearers) |
Primary amebic meningoencephalitis (seen primarily in immunocompetent patients) caused by Naegleria fowleri, granulomatous amebic encephalitis caused by Acanthamoeba spp. and Balamuthia; severe cutaneous infections in compromised patients (Acanthamoeba spp.) |
Giardia duodenalis (G. lamblia, G. intestinalis); often rotating between 2nd and 3rd most common organism with Dientamoeba fragilis. |
Asymptomatic to malabsorption syndrome |
Certain immunodeficiencies tend to predispose an individual to infection. |
Dientamoeba fragilis, often rotating between 2nd and 3rd most common organism with Giardia |
Asymptomatic to typical GI symptoms (abdominal pain, diarrhea) |
Not enough information to tell whether actual differences occur in compromised patients |
Blastocystis spp.Most common organism found in human fecal specimens (worldwide) morphology of pathogenic and nonpathogenic subtypes are the same and cannot be differentiated using routine microscopy |
Approximately 17 subtypes, half of which are pathogenic. Asymptomatic to symptomatic, often depending on subtypes and number of organisms present |
Not enough information to tell whether actual differences occur in compromised patients; infections may be more difficult to eradicate in AIDS patients; infections have been linked to irritable bowel syndrome and urticaria. |
Toxoplasma gondii |
Approximately 15% of individuals in the U.S. have antibody and organisms in tissue but are asymptomatic.; may be as high as >70% in some areas of Latin America |
Disease in compromised host tends to involve CNS, with various neurological symptoms |
Cryptosporidium spp. |
Self-limiting infection with diarrhea and abdominal pain |
Due to autoinfective nature of life cycle, will not be self-limiting, may produce fluid loss of over 10 liters/day, and may show multisystem involvement; no known totally effective therapy |
Cyclospora cayetanensis |
Self-limiting infection with diarrhea (3–4 days), with relapses common |
Diarrhea may persist for 12 wk. or more; biliary disease has also been reported for this group, particularly for those with AIDS. |
Cystoisospora belli |
Self-limiting infection with mild diarrhea or no symptoms |
May lead to severe diarrhea, abdominal pain, and possible death (rare case reports); diagnosis may occasionally be missed due to nonrecognition of oocyst stage; will not be seen when concentrated from PVA fixative |
Sarcocystis hominis, S. heydorni (beef) or S. suihominis (pork) |
Self-limiting infection with diarrhea or mild symptoms |
Symptoms may be more severe and last longer. |
Microsporidia (Brachiola, Nosema, Anncaliia, Vittaforma, Encephalitozoon, Enterocytozoon, Pleistophora, Trachipleistophora, Microsporidium spp.) |
Less is known about these infections in the healthy host; serological evidence suggests that infections may be more common than recognized. |
Can infect various parts of the body; diagnosis often depends on histological examination of tissues; routine examination of clinical specimens (stool, urine, etc.) is becoming more common; eye infections most common in contact lens wearers; NAAT also becoming more important; organisms can cause death |
Leishmania spp. |
Asymptomatic to severe, destructive, and even life-threatening disease |
More serious manifestations of visceral leishmaniasis; some cutaneous species will manifest visceral disease; difficult to treat and manage; definite coinfection with AIDS |
Strongyloides stercoralis |
Asymptomatic to mild abdominal complaints; can remain latent for many years due to low-level infection maintained by internal autoinfective life cycle |
Can result in disseminated disease (hyperinfection syndrome due to autoinfective nature of life cycle); abdominal pain, pneumonitis, sepsis-meningitis with Gram-negative bacilli, eosinophilia |
Scabies (Sarcoptes scabiei) |
Infections can range from asymptomatic to causing moderate itching. |
Severe infection with reduced itching response; hundreds of thousands of mites on body (crusted scabies); infection very easily transferred to others; secondary infection very common |
a CNS, central nervous system; PVA, polyvinyl alcohol; EM, electron microscopy.