About Medical Parasitology

New Infections

Ova & Parasite (O&P) Exams

CPT Codes

Quizzes, General

Quizzes, Histology

Quizzes, Blood

Review Tests


Information Tables

Organism Index (A-Z)

Presentation of Quiz #49

A 45-year-old naval engineer presented to his physician with swelling of his face and legs. He reported having diarrhea and general indigestion about two weeks before. He also indicated he had a bad upper respiratory infection about a month before. On the day he presented to the physician, during the discussion he commented that occasionally he saw undigested food in his stool. The patient works for an engineering firm that has multiple contracts throughout the Philippines, Taiwan, and Australia. During the previous six months, he had visited all of these areas, usually eating most of the local food specialties, including raw fish and raw vegetables served in salads. He had no underlying health problems.

On presentation, the patient appeared in no particular distress; however, there was some facial edema and pitting edema of the legs. The lungs and heart were normal.

His laboratory findings included a marked eosinophilia and leukocytosis. With his history of diarrhea, relevant travel, and eating habits, examinations for parasites revealed the following from the O&P examination:

This object measured about 140 by 70 microns and was easily seen in the saline wet preparation examination.

  1. Based on the patient's history and image seen above, what are the possibilities in terms of parasitic infections?
  2. Is there anything about this object that leads you to a definitive genus and species? If yes, how? If not, why not?

Scroll Down for Answer and Discussion







Answer and Discussion of Quiz #49

The image presented in Diagnostic Quiz #49 is the following:

  1. This image is a Fasciolopsis buski egg; these eggs generally measure 130 - 150 to 63 - 90 microns. They are unembryonated, operculated, large, ovoid, and usually brownish yellow in color. Because the eggs of several trematodes look alike, it was not possible visually to distinguish this egg from that of Fasciola hepatica and a few other trematode eggs. However, the history reveals key elements of an infection with Fasciolopsis buski.


The patient's symptoms will reflect the phase of the infection, as well as the number of parasites present in the host. In light infections, the adult worms inhabit the duodenum and jejunum; in heavy infections they may be found in the stomach and most of the intestinal tract. The attachment of worms to the mucosal wall produces local inflammation with hypersecretion of mucus, hemorrhage, ulceration, and possible abscess formation. In heavy infections, the worms may cause bowel obstruction, acute ileus, and absorption of toxic or allergic worm metabolites, producing general edema and ascites. Facial edema may occur as a result of hypoalbuminemia secondary to malabsorption or protein-losing enteropathy. A marked eosinophilia and leukocytosis are commonly seen.

Few symptoms are seen in light infections, but in heavier infections, the patient may have abdominal pain and diarrhea. In heavy infections, the stools are profuse and yellow-green, and contain increased amounts of undigested food. The symptoms may be confused with those of giardiasis, peptic ulcer, or other causes of bowel obstruction. Depending on the worm burden, the infection can be associated with severe wasting, prostration, and can lead to death.

Life Cycle:

Adult worms, which live in the small intestine of pigs and humans, produce unembryonated eggs that are passed into the environment with the feces. The eggs are operculated, large, ovoid, and yellow-brown. Depending on the temperature, the eggs embryonate within about 3 to 7 weeks. Once in the water, the mature miracidium hatches from the egg to infect the snail intermediate host; then it begins to develop into a first-generation sporocyst. Two generations of rediae are produced, and eventually cercariae are liberated from the snail. Cercariae encyst on water vegetation, such as water caltrops, water chestnuts, and water bamboo where they develop into metacercariae in about 4 weeks. Humans are infected by ingestion of uncooked aquatic vegetation on which metacercariae are encysted. Metacercariae excyst in the duodenum, attach to the duodenal or jejunal mucosa, and develop into adult worms within about 3 months.

Epidemiology and Control:

Reservoir hosts include dogs, pigs, and rabbits. The infection is commonly found in Bangladesh, Cambodia, central and southern China, India, Indonesia, Laos, Malaysia, Pakistan, Taiwan, Thailand, and Vietnam; infections have also been reported in Japan. Drainage of farm waste, use of manure for cultivation, and defecation in or near ponds or lakes that contain appropriate snails, with water plants acting as vectors, allows the life cycle to continue. To prevent the infection, plants should be cooked or immersed in boiling water for a few seconds before they are eaten or peeled. The use of unsterilized night soil for fertilizer should be prohibited.

Fasciolopsis buski Key Points - Laboratory Diagnosis

  1. Multiple stool specimens may be required in order to find the trematodes eggs; in a very light infection, eggs may not be recovered; however, the patient might be asymptomatic. Rarely adult worms are found during heavy infections.
  2. The eggs of Echinostoma ilocanum, Gastrodiscoides hominis, Fasciola hepatica, F. gigantica, and F. buski appear almost identical and are very difficult to differentiate from one another. When passed, the eggs contain undifferentiated embryos.
  3. The sedimentation concentration method should be used; because the eggs are operculated, they will not float using the zinc sulfate flotation concentration method. When adding iodine to the wet preparation, don't add too much or the eggs will stain very darkly and will resemble debris.
  4. The wet preparation can be examined using the 10X (low power) objective, these eggs are large enough that they can usually be seen using this magnification.
  5. Eggs of F. buski can resemble those of other trematodes mentioned above. The less mature the egg, the more difficult it may be to see the actual operculum - it blends into the shell outline (there are no opercular shoulders).
  6. Do not make the wet preparation too thick, if so, the eggs can be obscured by normal stool debris.

Treatment: Praziquantel is the drug of choice and can be given in a single dose of 40 mg/kg at bedtime. Another option would be albendazole at 400 mg twice a day for 3 days.

Three images of Fasciolopsis buski eggs from a routine stool examination (wet preparation). The first image is the egg seen above in a saline wet preparation (note the presence of the operculum at the top of the egg - hard to see). The middle image shows an egg photographed in a saline wet mount, but at a somewhat higher magnification (note the operculum at the upper end). The image on the far right shows an egg photographed from an iodine wet mount; the operculum is visible at the top of the egg.




  1. Garcia, LS, 2016. Diagnostic Medical Parasitology, 6th Ed., ASM Press, Washington, DC.
  2. Garcia, L.S. 2009. Practical Guide to Diagnostic Parasitology, 2nd Ed., ASM Press, Washington, D.C.


Each Quiz has a two section format: the first section will present the Quiz topic and the second section will provide a discussion of the answer and/or various options in response to the Quiz situation presented to the user. In some situations, there may be more than one correct response.

The content within this site is made possible through the extensive contribution of Lynne S. Garcia, M.S., MT(ASCP), CLS(NCA), BLM(AAB), F(AAM), Director, Consultantation and Training Services (Diagnostic Medical Parasitology and Health Care Administration). For additional information, she can be contacted at LynneGarcia2@verizon.net.

Reference: Garcia, L.S. 2015. Diagnostic Medical Parasitology, 6th Ed., ASM Press, Washington, D.C.

Quiz 1 Quiz 2 Quiz 3
Quiz 4 Quiz 5 Quiz 6
Quiz 7 Quiz 8 Quiz 9
Quiz 10 Quiz 11 Quiz 12
Quiz 13 Quiz 14 Quiz 15
Quiz 16 Quiz 17 Quiz 18
Quiz 19 Quiz 20 Quiz 21
Quiz 22 Quiz 23 Quiz 24
Quiz 25 Quiz 26 Quiz 27
Quiz 28 Quiz 29 Quiz 30
Quiz 31 Quiz 32 Quiz 33
Quiz 34 Quiz 35 Quiz 36
Quiz 37 Quiz 38 Quiz 39
Quiz 40 Quiz 41 Quiz 42
Quiz 43 Quiz 44 Quiz 45
Quiz 46 Quiz 47 Quiz 48
Quiz 49 Quiz 50 Quiz 51
Quiz 52 Quiz 53 Quiz 54
Quiz 55 Quiz 56 Quiz 57
Quiz 58 Quiz 59 Quiz 60
Quiz 61 Quiz 62 Quiz 63
Quiz 64 Quiz 65 Quiz 66
Quiz 67 Quiz 68 Quiz 69
Quiz 70 Quiz 71 Quiz 72
Quiz 73 Quiz 74 Quiz 75
Quiz 76 Quiz 77 Quiz 78
Quiz 79 Quiz 80 Quiz 81
Quiz 82 Quiz 83 Quiz 84
Quiz 85 Quiz 86 Quiz 87
Quiz 88 Quiz 89