The traditional Wright's stain dates from the early 1890's. The original Wright's stain was an alcoholic solution of methylene blue and eosin Y. Since then, there have been many modifications, most involving partial oxidative demethylation of the methylene blue to improve polychroming. Modern day samples of the dye usually contain mixtures of methylene blue, azure A, thionin and eosin Y. They also contain some amount of giemsa stain.
The traditional stain is diluted 1:1 with giordano buffer before use. Wright's One Step stain contains the buffer already dissolved in the stain. The slides are stained in the undiluted stain and differentiated by decolorizing in purified water.
|929A-16oz||Wright's One Step||16 oz.|
|929A-32oz||Wright's One Step||32 oz.|
|929A-1gl||Wright's One Step||1 gallon|
Note: Med Chem also manufactures a traditional Wright's stain (Cat#926A) and a Dip Stat procedure (Cat#300).
Freshly drawn blood that does not contain an anticoagulant is preferred. Fresh, EDTA treated blood may also be used but the anticoagulant will distort the cells. The sample should not be exposed to excessive heat. Hemolysis will render the sample unsatisfactory. Smears should be made in the usual way and air dried. Thin smears work best.
Rinse, air dry and examine stained smears under oil immersion in the usual manner. Staining times are approximate and should be varied as needed. Double the times for bone marrow smears.